R&D产品蛋白

  仪器信息网 ·  2011-03-24 00:46  ·  5606 次点击
R&D有超过1500种的天然或重组蛋白可供选择,包括:
lBCIP/NBT
l细胞因子Cytokines
l趋化因子Chemokines
l蛋白酶Proteases
l生长因子GrowthFactors
l粘附因子AdhesionMolecules
l神经营养因子NeurotrophicFactors
l干细胞因子StemCellFactors
l发育蛋白DevelopmentalProteins
l受体Receptors
lDNA修饰酶DNAModifyingEnzymes
l激酶、碳酸酶Kinases,Phosphatases,&Sulfatases
l抑制剂Inhibitors
与R&D抗体相同,严格的生产条件和QC保证了R&D蛋白具备以下诸多优点:
l高质量SuperiorQuality
l高纯度HighPurity(typically>95%)
l高生物活性HighBiologicalActivity
l低内毒素(LowEndotoxinLevel)
l批次间的一致性(HighLot-to-LotConsistency)
l不含叠氮钠NoNaN3andotherpreservatives
l高稳定性HighStability
What’s&WhyuseCF?
CF表示Carrier-free,代表蛋白产品不含携带物。
一般携带物是BSA(牛血清白蛋白),加入携带物是为了增强蛋白产品的稳定性,从而延长了产品的保存期限,加入比例为1μg的蛋白中加50μg的BSA。此外,含有携带物的产品,其重悬时,在不影响蛋白稳定性的前提下,可以得到更大的体积,更低的浓度,例如,含有携带物的产品,其浓度最低可以到10μg/ml,而CF(不含携带物)的产品,其浓度最低为100μg/ml。
但是,在有些实验中,如体内注射免疫生产抗体、自行标记蛋白或者包被多孔板等,携带物等额外蛋白的存在就会干扰实验。此时,只能使用CF(不含携带物)的产品。
大多数R&D蛋白产品都含/不含携带物两种产品形式可供选择。产品货号中含有“/CF”后缀的产品都是不含携带物的产品。
总的来说,含有携带物的产品,稳定性更佳,而CF(不含携带物)的产品应用范围更广泛,实验中大家可以根据具体实验要求,选择最适合实验需要的产品。
R&DSystemsoffersanextensiveselectionofpurifiedrecombinantandnatural:
Cytokines
Chemokines
Proteases
Growthfactors
Adhesionmolecules
Neurotrophicfactors
Developmentalproteins
Human,mouse,andratproteinsareourfocus.Inaddition,wehaveproteinsfromthefollowing:
Bovine,Equine,Feline,Porcine,Canine
Cottonrat,Chicken,Xenopus,Rhesusmacaque,Zebrafish
Drosophilaandotherinsects
Viralandbacterialproteins
通过以下三种方式检索您需要的蛋白:Locateyourproteinofinterestthreeways:
SearchforProducts–Indexcontainsanextensivelistofaliases
BrowsebyMolecule–Alphabeticallistingofanalytes
BrowsebyResearchTopic–Moleculesareorganizedintobiologicalprocessorfamiliesofinteresttovariousdisciplines
蛋白产品特点Ourproteinsfeature:
质量控制QualityControl
Eachnewlotistestedside-by-sidewithapreviouslotforbiologicalpotency,endotoxinlevel,andpurity.
Minimallot-to-lotvariability
生物活性BiologicalActivity
Measuredwithanappropriatebiologicalsystem
Testedtoensurebiologicalactivityisconsistentwithliterature-basedexpectations
纯度Purity
Recombinantproteinsaretypicallyover95%pure.
Ensuresthattheexperimentaloutcomeisaresultoftheproteinofinterestandnotduetothepresenceofacontaminant
内毒素水平EndotoxinLevel
EachnewproductionlotofproteinisassessedforendotoxinusingtheLimulusAmoebocyteLysates(LAL)assay.
Lowendotoxinlevelsareessentialwhenproteinsareusedinbiologicalsystemsthataresensitivetoitseffect.
形式Formulation
Mostrecombinantproteinsarelyophilizedfromasterilefilteredsolutioncontainingacarrierprotein,usuallybovineserumalbumin(BSA)at50microgramsper1microgramoftheproteinofinterest.
Givesthelyophilizedproteingreaterstability
Allowseasyhandlingandstorage
Maximizesrecoveryuponreconstitution
Forapplicationswherecarrierproteinisundesirable(e.g.invivoexperiments),carrier-freeproteinsareavailable(identifiablewiththecatalogextensionof"/CF").
Ourrecombinantenzymesareinsolutionandreadytouse.
特殊包装(大包装)BulkQuantities
Whenexperimentalprotocolsrequirelargequantitiesofaparticularprotein,specialbulkpacksizescanbepurchased.Thiswillreducethecostperunitmass.Pleasecontactusforabulkorderquote.
Animal-Free™RecombinantProteins
Tosupportdevelopingresearchandbioproductiondemands,weofferproductsmanufacturedinalaboratoryexclusivelydedicatedtotheproductionandpurificationofrecombinantproteinsusingallnon-animalreagents.Everystageofourestablishedfermentationandproteinpurificationmethodologieshasbeenspecificallymodifiedforthemanufactureofanimal-freeproducts.Animal-freeproteinsareimportantforresearchersconcernedwithexperimentalvariablescausedbytraceanimalcomponentsormammalianpathogensandareparticularlyimportantforexvivocelltherapystudies.Productsthataregeneratedunderanimal-freeconditionssharethesamebiologicalactivityasthoseproducedinatypicallaboratoryenvironment.
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